ABSTRACT
<p><b>OBJECTIVE</b>To explore the distribution of cytomegalovirus (CMV) in vascular tissues and the relationship between virus and atherosclerogenesis after CMV infecting mice.</p><p><b>METHODS</b>(1) C57 BL/6J Murine model of CMV infection was established by intraperitoneal injection of CMV lethiferous amount. (2) After 12 weeks of CMV infection, the sera, carotids, aorta, hearts and postcaval veins from the mice were collected under euthanasia. The tissues would be used to DNA extraction, PCR and pathological examination. (3) Interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in serum were measured with ILELISA.</p><p><b>RESULTS</b>(1) The typical pathologic feature in 2 aorta samples of 6 mice infected by CMV was found and the mice uninfected by CMV did not show any pathologic change. (2) CMV DNA appeared in 6 aorta, 6 postcaval veins, 4 carotids and 4 heart tissues including endocardium, cardiac muscle and coronary artery from the CMV infected mice. CMV DNA was not found in the vascular and heart tissues from 6 mice uninfected by CMV. (3) The ELISA test showed the significant difference (Mann-Witney test of Nonparametric Test, P < 0.05) in serum IL-6 (Median among 25% and 75% percentile: 113.7 pg/ml vs. 49.77 pg/ml) and MCP-1 (Median among 25% and 75% percentile: 128.7 pg/ml vs. 45.36 pg/ml) between CMV infected mice and uninfected mice.</p><p><b>CONCLUSION</b>Cardiovascular cells are CMV latent reservoir in host body and CMV infection and the cytokines induced by CMV infection probably relate to atherosclerogenesis.</p>
Subject(s)
Animals , Female , Humans , Mice , Atherosclerosis , Allergy and Immunology , Pathology , Virology , Blood Vessels , Allergy and Immunology , Pathology , Virology , Coronary Vessels , Allergy and Immunology , Pathology , Virology , Cytokines , Allergy and Immunology , Cytomegalovirus , Genetics , Cytomegalovirus Infections , Allergy and Immunology , Pathology , Virology , DNA, Viral , Genetics , Disease Models, Animal , Heart , Virology , Mice, Inbred C57BL , Random AllocationABSTRACT
<p><b>OBJECTIVE</b>To seek microstructure characters and method of insect material medicines.</p><p><b>METHOD</b>Insect material medicines and Chinese patent medicines containing these medicines were observed and compared with OLYMPUS.</p><p><b>RESULT</b>The texture of integumentary cells, the shape and size of hair socket and bristle were important characters for species identification.</p><p><b>CONCLUSION</b>This method can effectively identify 8 insect medicines, which provides some data and foundation for Chinese patent medicines' identification containing insects medicines and making quality standard for new drugs.</p>